简要描述:LightCycler ® EvoScript RNA 探针大师The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. Light
详细介绍
品牌 | 其他品牌 | 货号 | 07800029001 |
---|---|---|---|
规格 | reactions200x20µL | 供货周期 | 一个月以上 |
主要用途 | 基因表达分析 | 应用领域 | 医疗卫生,生物产业 |
LightCycler EvoScript RNA探针Master专为敏感、高特异性、高精度一步RT-qPCR而设计,特别适用于基因表达分析。
供一般实验室使用。
Product No:07800029001 产品编号:07800029001
Pack Size:200 x 20 µL reactions 包装尺寸:200 x 20 µL 反应
Product No:07800096001 产品编号:07800096001
Pack Size:1000 x 20 µL reactions 包装尺寸:1000 x 20 µL 反应
Keywords: Hydrolysis Probes 关键词: 水解探针
概述:
The LightCycler® EvoScript RNA Probes Master (1-vial composition) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
LightCycler®EvoScript RNA Probes Master(1 瓶组合物)专为灵敏、高特异性、高精度的一步法 RT-qPCR 而设计,尤其适用于基因表达分析。
Ease of use, convenience, speed, reduced error
Result consistency, stability and tolerance of targets of varying secondary structure
Ability to detect low copy or rare transcripts for gene expression applications
Superior quantification capability for gene expression applications
Easier assay optimization
Contamination control capability
Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting
Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction
Obtain fast and specific results
Contents
Master Mix, 5× conc. Contains enzymes for reverse transcription and PCR, RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, and dUTP, Mg(OAc)2, and proprietary additive
07 800 029 001 2 vials, 440 μL each
07 800 096 001 10 vials, 440 μL each
Water, PCR grade To adjust the final reaction volume.
07 800 029 001 3 vials, 1 mL each
07 800 096 001 2 vials, 7.25 mL each
内容
应用:
The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers, probe and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR.
LightCycler EvoScript RNA探针Master是专为敏感、高特异性、高精度一步RT-qPCR而设计的,特别适用于基因表达分析。1瓶的组合物非常适合简单的反应组装,只需要添加引物、探针和目标RNA。专有的反应缓冲液允许方便的热启动RT-qPCR。
In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1).
The master mix is optimized for hydrolysis probes, including Universal ProbeLibrary (UPL) probes and does not require optimization with Mg(OAc)2.
There are protocols for both the LightCycler® 480 instrument and the LightCycler® 96 instrument.
1) The reverse transcription activity will be suppressed at temperatures significantly below 60°C.
此外,用于逆转录和 PCR 的酶具有热启动特性,具有的特异性1)。
预混液针对水解探针进行了优化,包括 Universal ProbeLibrary (UPL) 探针,不需要使用 Mg(OAc) 2进行优化。
LightCycler ® 480 仪器和 LightCycler都有协议® 96 仪器。
1) 显着低于 60°C 的温度会抑制逆转录活性。
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