LightCycler ® EvoScript RNA 探针大师

The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. 

LightCycler EvoScript RNA探针Master专为敏感、高特异性、高精度一步RT-qPCR而设计，特别适用于基因表达分析。 

供一般实验室使用。

Product No：07800029001   产品编号：07800029001   

Pack Size：200 x 20 µL reactions  包装尺寸：200 x 20 µL 反应

Product No：07800096001  产品编号：07800096001   

Pack Size：1000 x 20 µL reactions   包装尺寸：1000 x 20 µL 反应

Keywords: Hydrolysis Probes  关键词： 水解探针

概述：

The LightCycler® EvoScript RNA Probes Master (1-vial composition) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

LightCycler®EvoScript RNA Probes Master（1 瓶组合物）专为灵敏、高特异性、高精度的一步法 RT-qPCR 而设计，尤其适用于基因表达分析。

Ease of use, convenience, speed, reduced error

Result consistency, stability and tolerance of targets of varying secondary structure

Ability to detect low copy or rare transcripts for gene expression applications

Superior quantification capability for gene expression applications

Easier assay optimization

Contamination control capability

Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting

Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction

Obtain fast and specific results

•  
• 适用简单、方便快捷、减少错误
• 结果表明，不同二级结构的目标具有一致性、稳定性和耐受性
• 能够检测低拷贝或罕见的转录本的基因表达应用
• 基因表达应用定量能力
• 更容易分析优化
• 污染控制能力
• 生成一致且可靠的结果，无论您从什么样本类型开始或检测什么指标
• 在检测低拷贝数时依靠更高的灵敏度，将更大的样品体积输入到反应中
• 获得快速和具体的结果

Contents

Master Mix, 5× conc. Contains enzymes for reverse transcription and PCR, RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, and dUTP, Mg(OAc)2, and proprietary additive

07 800 029 001   2 vials, 440 μL each

07 800 096 001 10 vials, 440 μL each

Water, PCR grade To adjust the final reaction volume.

07 800 029 001   3 vials, 1 mL each

07 800 096 001   2 vials, 7.25 mL each

内容

• 反应混合物，5× conc.包含逆转录和PCR酶，RT-qPCR反应缓冲酶，dATP, dCTP, dGTP, dUTP, Mg(OAc)2和专有添加剂
• 07 800 029 001    2瓶，每瓶 440 μL
  07 800 096 001    10瓶，每瓶440μL

• 水，PCR 级 调整最终反应体积。
  07 800 029 001    3瓶，每瓶 1 mL
  07 800 096 001    2瓶，每瓶 7.25 mL

应用：

The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers, probe and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR.

LightCycler EvoScript RNA探针Master是专为敏感、高特异性、高精度一步RT-qPCR而设计的，特别适用于基因表达分析。1瓶的组合物非常适合简单的反应组装，只需要添加引物、探针和目标RNA。专有的反应缓冲液允许方便的热启动RT-qPCR。

In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1).

The master mix is optimized for hydrolysis probes, including Universal ProbeLibrary (UPL) probes and does not require optimization with Mg(OAc)2.

There are protocols for both the LightCycler® 480 instrument and the LightCycler® 96 instrument.

1) The reverse transcription activity will be suppressed at temperatures significantly below 60°C.

此外，用于逆转录和 PCR 的酶具有热启动特性，具有的特异性1)。

预混液针对水解探针进行了优化，包括 Universal ProbeLibrary (UPL) 探针，不需要使用 Mg(OAc) 2进行优化。

LightCycler ®  480 仪器和 LightCycler都有协议®  96 仪器。

1) 显着低于 60°C 的温度会抑制逆转录活性。